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© 2000 Society of Cosmetic Chemists
Journal of the Society of Cosmetic Chemists, Vol. 51, No. 3, 193-203


Investigations on the penetration of hydrolyzed wheat proteins into human hair by confocal laser-scanning fluorescence microscopy
J. A. Swift , S. P. Chahal , N. I. Challoner , J. E. Parfrey

ABSTRACT

Synopsis

Hydrolyzed wheat proteins, of a type commonly used in hair toiletry products, were labeled by reaction with fluorescein isothiocyanate. Aqueous solutions were applied to untreated hair and also to hairs treated beforehand by permanent waving, by bleaching, or by relaxers. The extent of penetration of the peptides into the hairs and their structural locations in them were determined by imaging transverse sections of the resin-embedded hairs with a confocal laser-scanning fluorescence microscope. Experimental evidence and arguments are mustered that the fluorescently labeled peptides, having been purified, provide a satisfactory model for studying the diffusional behavior of the native peptides in hair. Penetration of the hydrolyzed wheat proteins into all the hairs was extensive. The main sites for occupation of the peptides were the endocuticle and in the cortex, the nuclear remnants (very intense), intermacrofibrillar matrix, and along the cell boundaries.

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© 2000 Society of Cosmetic Chemists
Journal of the Society of Cosmetic Chemists