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© 1995 Society of Cosmetic Chemists
Journal of the Society of Cosmetic Chemists, Vol. 46, No. 1, 39-52


A simple and sensitive method using protein loss measurements to evaluate damage to human hair during combing
Sukhvinder S. Sandhu , Ram Ramachandran , Clarence R. Robbins

ABSTRACT

Synopsis

A simple method to quantify hair damage during combing or brushing has been developed. The method involves collecting hair fragments that are chipped from hair during combing and quantitatively measuring the amount of protein using a colorimetric procedure capable of detecting as little as 5 μg of protein per ml. Using this procedure on hair tresses in the laboratory and on live heads (half-head tests), we were able to demonstrate significant differences in protein loss during post-shampoo combing of undamaged and chemically damaged hair previously treated with various shampoos\/conditioners. The method is applicable to different types of hair tested, namely, Caucasian, Asian, Oriental and Negroid.

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© 1995 Society of Cosmetic Chemists
Journal of the Society of Cosmetic Chemists