Squamometry and corneosurfametry for rating interactions of cleansing products with stratum corneum
G. E. Piérard , V. Goffin , C. Piérard-Franchimont

Synopsis

Interactions between cleansing products and human stratum corneum are complex and not fully understood. In this study we have compared data gained by two in vitro methods called squamometry (SQM) and corneosurfametry (CSM). These concern colorimetric measurements of human stratum corneum stained with basic fuchsin-toluidine blue solution (PMS) after contact of corneocytes with diluted solutions of cleansing agents. The interaction between surfactants and corneocytes induced alterations in the intensity of PMS staining of stratum corneum that were evaluated by color differentials DE*ab. The superficial stratum disjunctum response to surfactants, which is evaluated by SQM, was different from that of the mid-part of the stratum corneum as evaluated by CSM. Both methods appear valuable and complementary in the comparison of human skin compatibility of personal care cleansing products.

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