An improved procedure for quantitative analysis of sebum production using Sebutape®
A. Pagnoni , A. M. Kligman , S. el Gammal , C. Popp , T. Stoudemayer

Synopsis

Sebutape® is an adhesive, white tape specifically designed for the collection of sebum. As sebum issues from the orifices, it is trapped in microcavities in the tape, yielding a pore pattern that can be quantified in regard to the number of spots, total area, and size distribution. We have become aware of substantial alterations in pore patterns when Sebutape® is stored for later analysis. These changes relate to enlargement of spots, decrease in their transparency, and increased translucency of the tape itself.

We analyzed the evolution of these changes over time and in different storage conditions. We found that placing the Sebutape® on a plastic sheet and storing it in the freezer largely prevented these alterations. Room temperature storage is acceptable only if the samples are evaluated within 24 hours. Analysis immediately after removal yields the least artefacts. Recognizing the importance of changes associated with storage enhances the reliability and accuracy of the Sebutape® method.

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