Use of microspectrophotometry in dermatological investigations
Gary L. Grove , Robert M. Lavker , Albert M. Kllgman

Synopsis

Quantitative estimation of dimensions of structure and amounts of material in skin at the light microscope level has, until now, required time-consuming and tedious methods that are often subject to observer error. We have overcome these problems by using a Vickers M86 scanning-integrating microspectrophotometer. This analytical light microscope detects the amount of light which can pass through a specimen and then electronically converts this value into units of absorbance and projected area. This approach is very versatile and is in fact applicable to any biological structure which can be identified at the light microscope level and in which an appropriate change in color intensity can be realized. The fundamental principles of visible light MICROSPECTROPHOTOMETRY and its application to DERMATOLOGICAL STUDIES that objectively evaluate the pathophysiological status of skin are described.

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